Binding of Specific Soluble Ribonucleic Acid to Ribosomes
نویسندگان
چکیده
منابع مشابه
Comparative studies on specific and nonspecific binding of transfer ribonucleic acid to ribosomes.
The binding of aminoacyl transfer ribonucleic acid @RNA) to ribosomes in the presence of corresponding messenger RNA (specific binding) is compared with that in the absence of messenger RNA (nonspecific binding). Specific binding is much more sensitive to tetracycline than nonspecific binding, and the former is similar to, but not identical with, the latter in its response to changes in Mg++ co...
متن کاملDemonstration of a guanosine triphosphate-dependent enzymatic binding of aminoacyl-ribonucleic acid to Escherichia coli ribosomes.
Multiple enzyme fractions have been shown to be required for the synthesis of polypeptides from aminoacyl-sRNA in both mammalian and microbial systems.'-6 In the reticulocyte system two enzyme fractions have been found to be required for the polyuridylic acid-(poly U)-directed synthesis of polyphenylalanine.7 One of these fractions was found to enhance the binding of phenylalanyl-sRNA to reticu...
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The MO-formyltetrahydrofolate:methionyl soluble ribonucleic acid transformylase has been purified over 1500-fold from Escherichia coli B. Although not homogeneous at this stage, the enzyme was of sufficient purity to determine some physical characteristics. The sedimentation constant was 2.02 which would indicate a molecular weight of approximately 25,000. In addition to the substrates of the r...
متن کاملBinding of Amino Acids to the End Group of a Soluble Ribonucleic Acid.
Introduction.-One of the intermediate steps in the process of protein synthesis appears to be the formation of a RNA-amino acid compound.'-" The acceptors of the amino acids are low molecular weight RNA molecules, which contain a specific nucleotide end grouping consisting of one or two cytosine nucleotides and a terminal adenine nucleotide."1 Terminal addition of nucleotides to RNA has been ob...
متن کاملIntermediate reactions in the binding of aminoacyl-transfer ribonucleic acid to rat liver ribosomes. Formation and properties of an aminoacyl-transfer ribonucleic acid-transferase I complex.
1. Transferase I of rat liver binds aminoacyl-tRNA to form a relatively stable complex, which is retained on cellulose nitrate filters. This reaction proceeds at both 0 degrees C and 37 degrees C and is inhibited by GTP. The resulting product is stabilized by GTP and Mg(2+). 2. Only very low quantities of deacylated tRNA are bound by transferase I. 3. Methods are described for the preparative i...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96767-0